Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Dec 7, 2019; 25(45): 6619-6633
Published online Dec 7, 2019. doi: 10.3748/wjg.v25.i45.6619
Figure 1
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Figure 1 The expression of microRNA-760 is decreased in gastric cancer. A: MicroRNA-760 (miR-760) expression in gastric cancer tissues; B: MiR-760 expression in MKN-45, AGS, and GES-1 cells; C and D: Dysregulation of miR-760 was related to overall survival and disease-free survival in GC patients. bP < 0.01. GC: Gastric cancer; MiR-760: MicroRNA-760.
Figure 2
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Figure 2 MicroRNA-760 regulates cell growth and apoptosis in gastric cancer. A: MicroRNA-760 (miR-760) expression in MKN-45 and AGS cells transfected with its mimic or inhibitor; B-D: Cell proliferation and colony formation regulated by miR-760 mimic or inhibitor; E: Flow cytometry analysis of MKN-45 and AGS cells transfected with miR-760 mimic or inhibitor. aP < 0.05, bP < 0.01. NC: Negative control; MiR-760: MicroRNA-760.
Figure 3
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Figure 3 MicroRNA-760 suppresses G-protein-coupled receptor kinase interacting protein-1 expression in gastric cancer. A: The binding site between microRNA-760 (miR-760) and G-protein-coupled receptor kinase interacting protein-1 (GIT1); B: Luciferase reporter assay; C: A negative correlation between miR-760 and GIT1 in gastric cancer tissues; D and E: GIT1 expression in MKN-45 and AGS cells transfected with miR-760 mimic or inhibitor. bP < 0.01. GC: Gastric cancer; Mut: Mutant; Wt: Wild-type; 3'-UTR: 3'-untranslated region; NC: Negative control; MiR-760: MicroRNA-760; GIT1: G-protein-coupled receptor kinase interacting protein-1.
Figure 4
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Figure 4 G-protein-coupled receptor kinase interacting protein-1 is upregulated in gastric cancer. A: G-protein-coupled receptor kinase interacting protein-1 (GIT1) expression in gastric cancer tissues; B: GIT1 expression in MKN-45, AGS, and GES-1 cells; C and D: High GIT1 expression was related to a shorter overall survival and disease-free survival in gastric cancer patients. bP < 0.01. GIT1: G-protein-coupled receptor kinase interacting protein-1.
Figure 5
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Figure 5 MicroRNA-760 inhibits gastric cancer progression by inhibition of G-protein-coupled receptor kinase interacting protein-1. A: G-protein-coupled receptor kinase interacting protein-1 (GIT1) expression in MKN-45 and AGS cells transfected with GIT1 vector and microRNA-760 (miR-760) mimic; B-D: Cell proliferation and colony formation in MKN-45 and AGS cells transfected with GIT1 vector and miR-760 mimic; E: Flow cytometry analysis of MKN-45 and AGS cells transfected with GIT1 vector and miR-760 mimic. bP < 0.01. NC: Negative control; MiR-760: MicroRNA-760; GIT1: G-protein-coupled receptor kinase interacting protein-1.
Figure 6
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Figure 6 Relevance of microRNA-760/G-protein-coupled receptor kinase interacting protein-1 axis in human gastric cancer. A: High expression of microRNA-760 was associated with a good prognosis in gastric cancer patients; B: G-protein-coupled receptor kinase interacting protein-1 (GIT1) expression in gastric cancer tissues; C: High expression of GIT1 was associated with a poor prognosis in gastric cancer patients; D: Up-regulation of GIT1 was related to cell proliferation and apoptosis in gastric cancer cells. HR: Hazard ratio; MiR-760: MicroRNA-760.