Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer

Figure 1 High expression of Wnt1-inducible signaling pathway protein 1 mRNA in gastric cancer predicted based on Oncomine and Coexpedia databases. A: Meta-analysis of 17 datasets from five studies on Wnt1-inducible signaling pathway protein 1 (WISP1) mRNA levels in gastric cancer (GC) vs normal gastric tissue based on the Oncomine database; B: WISP1 mRNA levels of GC vs normal gastric tissue in Coexpedia databases from The Cancer Genome Atlas (TCGA) (P < 0.05); C: The expression of WISP1 mRNA was associated with clinical stage in Coexpedia database from TCGA (P < 0.05). WISP1: Wnt1-inducible signaling pathway protein 1; TCGA: The Cancer Genome Atlas; STAD: Stomach adenocarcinoma.

Figure 2 Expression of Wnt1-inducible signaling pathway protein 1 at the mRNA and protein levels is upregulated in gastric cancer patients. A: Western blot analysis showed the expression of Wnt1-inducible signaling pathway protein 1 (WISP1) at the protein level in 20 samples; B: Negative expression of WISP1 in gastric cancer by immunohistochemical staining; C: Positive expression of WISP1 in gastric cancer by immunohistochemical staining; D: Statistical results of WISP1 expression at the mRNA level in 20 samples; E: Statistical results of WISP1 expression at the protein level in 20 samples; F: Statistic results of the immunohistochemical staining for WISP1 at the protein level in 20 samples. WISP1: Wnt1-inducible signaling pathway protein 1.

Figure 3 Wnt1-inducible signaling pathway protein 1 expression is associated with overall survival in gastric cancer. A: Kaplan-Meier survival curves plotted with Coexpedia by stratifying patients into high and low Wnt1-inducible signaling pathway protein 1 (WISP1) expression groups based on the median expression value at the mRNA level; B: Kaplan-Meier survival curves generated by WISP1 expression at the protein level. CI: Confidence interval; HR: Hazard ratio; WISP1: Wnt1-inducible signaling pathway protein 1.

Figure 4 Mechanistic role of Wnt1-inducible signaling pathway protein 1 in gastric cancer cells. A and B: Silencing of Wnt1-inducible signaling pathway protein 1 (WISP1) has effects on cell viability of MKN45 and AGS cells; C: Silencing of WISP1 results in a significant decrease in the IC50 value; D and E: Silencing of WISP1 results in γH2AX overexpression and XRCC1 downregulation in MKN45 and AGS cells. NC: The matching scramble control siRNAs were transfected into cell lines as normal controls; RNAi: The siRNAs were transfected into cell lines as RNA interference; WISP1: Wnt1-inducible signaling pathway protein 1.