Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR-1273a

Figure 1 Expression of circPIP5K1A in colon cancer. A: RT-qPCR detection of circPIP5K1A expression in tumors and their adjacent normal tissues; B: The mRNA level of circPIP5K1A in various normal and cancer cell lines; C: The circPIP5K1A level in COLO320DM cells with different degrees of confluence. ^aP < 0.01 vs 80% or 100% group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A.

Figure 2 Silencing of circPIP5K1A promotes cell death. A: RT-qPCR detection of circular RNA PIP5K1A expression in COLO320DM cells transfected with siRNA1, siRNA2, or siRNA3 in the absence or presence of RNase R; B: RT-qPCR detection of PIP5K1A mRNA level in COLO320DM cells transfected with siRNA1, 2, and 3 in presence or absence of RNase R; C: Cell death rate in COLO320DM cells transfected with siRNA1, 2, or 3 by Hoechst staining. ^aP < 0.05, ^bP < 0.01 vs oligo or control group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A; siRNA: Small interfering RNAs.

Figure 3 Role of circPIP5K1A in the modulation of cell migration. The migratory function of COLO320DM cells transfected with circPIP5K1A vector or siRNA1, siRNA2, or siRNA3 was measured by the Transwell assay. ^aP < 0.05, ^bP < 0.01 vs control group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A; siRNA: Small interfering RNAs.

Figure 4 Effect of miR-1273a overexpression on circPIP5K1A upregulation. A: Binding site of miR-1273a and circPIP5K1A; B: Expression of AP-1, IRF-4, CDX-2, and Zic-1 in COLO320DM cells transfected with circPIP5K1A vector, miR-1273a vector, or co-transfection of circPIP5K1A vector and overexpressing miR-1273a vector, respectively, by western blotting. ^aP < 0.05, ^bP < 0.01 vs control group; ^cP < 0.05, ^dP < 0.01 vs circPIP5K1A group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A; AP-1: Activator protein 1; IRF-4: Interferon regulating factor 4; CDX-2: Caudal type homeobox 2; Zic-1: Zinc finger of the cerebellum 1.