High expression of APC is an unfavorable prognostic biomarker in T4 gastric cancer patients

Figure 1 Prognostic value of APC expression in gastric cancer patients. A: Overall survival of gastric cancer patients with high APC expression and those with low expression (based on median value); B: Relapse-free survival of gastric cancer patients with high APC expression and those with low expression; C: Distribution of APC expression; D: Relapse-free survival of T4 gastric cancer patients with high APC expression and those with low expression. APC: Adenoma polyposis coli; GC: Gastric cancer.

Figure 2 Differentially expressed mRNAs associated with APC^high. A: Volcanic map of differentially expressed mRNAs associated with APC^high; (B) Heatmap of differentially expressed mRNAs associated with APC^high. APC: Adenoma polyposis coli.

Figure 3 KEGG pathways of differentially expressed mRNAs associated with APC^high. Upregulated pathways of (A) cell receptor signaling and (B) pancreatic cancer and downregulated pathways of (C) ascorbate and aldarate metabolism and (D) PPAR signaling pathway are shown. APC: Adenoma polyposis coli.

Figure 4 Genome-wide analysis of miRNAs associated with APC^high. A: Relationship between the correlation coefficient and correlation P-value; B: MiRNAs that were significantly positively correlated (red), negatively correlated (green), and had no correlation (blue) with APC^high, as well as the heatmap of miRNAs associated with APC^high; C: mRNA-miRNA regulatory network. APC: Adenoma polyposis coli.

Figure 5 Differential expression of DNMT1, DNMT3A, and DNMT3B between APC^high and APC^low. APC: Adenoma polyposis coli; DNMT: DNA methyltransferase.

Figure 6 Analysis of relationship between APC^high and genome-wide DNA methylation. A: Volcanic map of differential DNA methylation associated with APC^high; B: Distribution of DNA methylation associated with APC^high based on CPG islands; C: Distribution of DNA methylation associated with APC^high based on transcription initiation site; D: Upregulated KEGG pathways of genes corresponding to the nearest transcription initiation site of differential DNA methylation; E: Downregulated KEGG pathways of genes corresponding to the nearest transcription initiation site of differential DNA methylation. APC: Adenoma polyposis coli.