Clinical assessment and identification of immuno-oncology markers concerning the 19-gene based risk classifier in stage IV colorectal cancer

Figure 1 Progression-free survival in 60 patients with colorectal cancer, classified according to the 19-gene based risk score and chemotherapy regimens. A: Progression-free (PFS) survival graph in the patients with higher 19-gene based risk (TCA19) score. B: PFS survival graph in the patients with lower TCA19 score. PFS: Progression-free survival; 5-FU: Fluorouracil-based regimen; FOLFOX: Combined regimen of fluorouracil, leucovorin, and oxaliplatin; FOLFIRI: Combined regimen of fluorouracil, leucovorin, and irinotecan; Beva/Cetux: Target-regimen including bevacizumab and cetuximab.

Figure 2 Expression of CD68 in a signaling lymphocytic activation molecule family, member 7-expression colorectal cancer cell line. High-through imaging and results of CD68(+)/signaling lymphocytic activation molecule family, member 7 (SLAMF7)(+) at day 0 and day 5 of co-culture with THP-1 monocytes, THP-1 M2 macrophages, and lipopolysaccharide activated THP-1. On day 5, expression of CD68 was significantly lower than day 0 in the SLAMF7-expressing region. SLAMF7: Signaling lymphocytic activation molecule family, member 7; LPS: Lipopolysaccharide; CRC: Colorectal cancer.

Figure 3 Expression of CD73 in a signaling lymphocytic activation molecule family, member 7-expression colorectal cancer cell line. High-through imaging and results of CD73(+)/signaling lymphocytic activation molecule family, member 7 (SLAMF7)(+) at day 0 and day 5 of co-culture with THP-1 monocytes, THP-1 M2 macrophages, and lipopolysaccharide activated THP-1. On day 5, expression of CD73 was significantly lower than day 0 in the SLAMF7-expressing region. SLAMF7: Signaling lymphocytic activation molecule family, member 7; LPS: Lipopolysaccharide; CRC: Colorectal cancer.