Dysregulation of PARP1 is involved in development of Barrett’s esophagus

Figure 1 Establishment of a Barrett’s esophagus mouse model. A: HE staining; B: γH2AX staining; C: NF-κB staining; D: Poly(ADP-ribose) staining.

Figure 2 PARP1 is aberrantly expressed in Barrett's esophagus. A: Microarray profiling of gene expression between esophageal squamous epithelium and Barrett's esophagus; B: PARP1 is overexpressed in Barrett's esophagus and Barrett's esophagus cell lines; C: H₂O₂ increased PARP1 expression; D: BAs (pH 4) increased PARP1 expression.

Figure 3 PARP1 protects Barrett's esophagus cells from H₂O₂-induced oxidative damage. A: Knockdown of PARP1 in BAR-T cells; B: BAR-T cells were more sensitive to H₂O₂-induced cell death after PARP1 knockdown; C: BAR-T cells were more sensitive to H₂O₂-induced DNA damage after PARP1 knockdown.

Figure 4 PARP1 protects Barrett's esophagus cells from BA (pH 4)-induced oxidative damage. A: H₂O₂ levels in PARP1-depleted BAR-T cells; B: 8-OXOG staining in PARP1-depleted BAR-T cells; C: γH2AX staining in PARP1- depleted BAR-T cells; D and E: Decreased cell viability in PARP1-depleted BAR-T cells.