Pharmacokinetics and pharmacodynamics of Shengjiang decoction in rats with acute pancreatitis for protecting against multiple organ injury

Figure 1 Chemical structure of the major components of Shengjiang decoction detected in the serum and pancreas. A: Emodin; B: Aloe-emodin; C: Rhein; D: Chrysophano; E: Curcumin; F: Demethoxycurcumin; G: Bisdemethoxycurcumin.

Figure 2 HPLC chromatograms of the major components of Shengjiang decoction detected in the serum and pancreas. A: Emodin; B: Aloe-emodin; C: Rhein; D: Chrysophanol; E: Curcumin; F: Demethoxycurcumin; G: Bisdemethoxycurcumin; H: Internal standard (I.S.).

Figure 3 Estimated concentration-time curves of seven components in the two groups. Twelve male Sprague-Dawley rats were randomly divided into a control group treated with SJD (CG + SJD) and a model group treated with SJD (MG + SJD), both of which were orally administered with SJD (5 g/kg) 2 h after surgery. Blood samples were collected via the tail vein at 10, 20, and 40 min and 1, 2, 3, 4, 6, 8, and 12 h after a single dose of SJD to detect its main components.

Figure 4 Amylase and lipase concentrations of the control group, model group, and Shengjiang decoction group. Rats were randomly divided into a control group (CG), an AP model group (MG), and an SJD treated AP group (SJDG) (n = 6 per group). The rats were sacrificed 12 h after administration of SJD. Blood samples were collected to obtain serum samples to measure amylase and lipase. Data are expressed as mean ± SD. ^aP < 0.05 vs CG.

Figure 5 Serum concentrations of IL-6, IL-10, and TNF-α in the control group, model group, and Shengjiang decoction group. Rats were randomly divided into a control group (CG), an AP model group (MG), and an SJD treated AP group (SJDG) (n = 6 per group). The rats were sacrificed 12 h after administration of SJD. Blood samples were collected to obtain serum samples to measure IL-6, IL-10, and TNF-α. Data are expressed as mean ± SD. ^bP < 0.01 vs CG; ^dP < 0.01 vs MG.

Figure 6 Pathological scores of multiple organs in the control group, model group, and Shengjiang decoction group. Rats were randomly divided into a control group (CG), an AP model group (MG), and an SJD treated AP group (SJDG) (n = 6 per group). The rats were sacrificed 12 h after administration of SJD. Tissue samples were collected for pathological examination. Data are expressed as mean ± SD. ^aP < 0.05 vs CG; ^bP < 0.01 vs CG; ^cP < 0.05 vs MG; ^dP < 0.01 vs MG.

Figure 7 Pathological images of multiple organs from the control group, model group, and Shengjiang decoction group. Pathological images of the heart (HE, × 200), lung (HE, × 200), liver (HE, × 100), spleen (HE, × 100), pancreas (HE, × 200), kidney (HE, × 200), and intestine (HE, × 100) in the CG, MG, and SJDG. CG: Control group; MG: AP model group; SJDG: SJD treated AP group.