Genetic polymorphism in CD14 gene, a co-receptor of TLR4 associated with non-alcoholic fatty liver disease

Figure 1 Polymorphism analysis of C14-159C/T. A: Representative PAGE gel picture of PCR-RFLP assay with restriction digested products. Lane M: 100 bp DNA ladder; Lanes 1-8: RE digested product, CC: 141 bp and 154 bp (Lanes 2, 8), CT: 296 bp, 141 bp, and 154 bp (Lanes 4 and 5), TT: 296 bp (Lanes 1, 3, 6, and 7); Lanes 9-11: PCR product; B: DNA sequencing showing different genotypes. Gap indicates the SNP or mutation (C→T). wt: Homozygous wild; mt: Homozygous or heterozygous mutant; PCR: Polymerase chain reaction; RFLP: Restriction fragment length polymorphism.