Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation

Figure 1 Flow diagram of the study design. DSS: dextran sulfate sodium; F. prausnitzii: Faecalibacterium prausnitzii.

Figure 2 Body weight and colonic length in mice. A, B: Body weight change; C, D: Colon length. Data are the mean ± SD. n = 8-10. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs model group.

Figure 3 Proportion of Th17 cells in splenic mononuclear cells and plasma cytokines levels. Flow cytometry figures (A) and statistical analysis (B) of Th17 cell in each group of the mice splenic MNC. Plasma IL-17 A (C), IL–6 (D) and IL-4 (E) levels by enzyme-linked immunosorbent assay. Data are the mean ± SD. n = 8-10. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs model group. IL: Interleukin; MNC: mononuclear cells.

Figure 4 Cytokine mRNA expression in colon mucosal tissue. A: RORγt mRNA; B: IL-17A mRNA; C: IL-6 mRNA. Data are the mean ± SD. n = 8-10. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs model group. ROR-γt: Related orphan receptor-γt; IL: Interleukin.

Figure 5 Colon Neurath Scores and related orphan receptor-γt and interleukin-17A protein expression. Colon Neurath Scores (A, 100 magnifications), RORγt (B, 200 magnifications), and IL-17A (C, 200 magnifications) protein expression in mice colon. Representative images of mice colonic mucosa (1a-1d). Representative immunohistochemical staining of RORγt (2a-2d) and IL-17A (3a-3d) in mice colon mucosa. Control group (a); model group (b); treatment group (c); prevention group (d). Data are the mean ± SD. n = 8-10. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs model group.