Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. May 28, 2016; 22(20): 4868-4880
Published online May 28, 2016. doi: 10.3748/wjg.v22.i20.4868
Figure 1
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Figure 1 Expression of hypoxia inducible factor-1 alpha and glycolysis enzymes at different time points of hypoxia in Eca109 cells (A) or in TE13 cells (B). HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.
Figure 2
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Figure 2 Lactic levels in culture medium of Eca109 cells (A) or TE13 cells (B) in different oxygen environments and at different time points as revealed by photocolorimetric method.
Figure 3
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Figure 3 Quantitative real-time PCR analysis of the gene expression of hypoxia inducible factor-1 alpha and glycolysis (n = 3). HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.
Figure 4
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Figure 4 Effect of wortmannin on hypoxia inducible factor-1 alpha and glycolysis protein in Eca109 cells (n = 3) (A) and in TE13 cells (B) (n = 3). HIF-1α: Hypoxia inducible factor-1 alpha; HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.
Figure 5
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Figure 5 Spectrophotometry analysis of the activities of lactate dehydrogenase and hexokinase (n = 3) (A) or the supernatant lactic acid concentration in nutrient solution (n = 3) (B). HK: Hexokinase; LDH: Lactate dehydrogenase.
Figure 6
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Figure 6 mRNA expression of glycolysis enzymes after hypoxia inducible factor-1 alpha inhibition (n = 3). 1: Eca109; 2: Eca109/neo; 3: Eca109/siRNA. HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.
Figure 7
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Figure 7 Protein expression of glycolysis enzymes. A: In Eca109 cells after HIF-1α inhibition (n = 3). N: Eca109 + 19% O2; N + iRNA: Eca109/siRNA + 19% O2; H: Eca109 + 1% O2; H + iRNA: Eca109/siRNA + 1% O2; B: In TE13 cells after HIF-1α inhibition (n = 3). N: TE13 + 19% O2; N + iRNA: TE13/siRNA + 19% O2; H: TE13 + 1% O2; H + iRNA: TE13/siRNA + 1% O2. HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.
Figure 8
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Figure 8 Western blot analysis of the activities of glycolysis enzymes after hypoxia inducible factor-1 alpha inhibition. HIF-1α: Hypoxia inducible factor-1 alpha; HK-II: Hexokinase II; GLUT-1: Glucose transporter-1; LDHA: Lactate dehydrogenase-A.