Pathophysiology of chronic pancreatitis induced by dibutyltin dichloride joint ethanol in mice

Figure 1 Morphological changes in pancreatic sections obtained from mice with chronic pancreatitis induced by Dibutyltin Dichloride joint Ethanol drinking. A: The pancreatic morphology at different time-point by HE staining (original magnification: 100 ×); B: Collagen deposition by Masson staining (original magnification: 200 ×).

Figure 2 Biochemical parameter changes in serum of mice with chronic pancreatitis induced DBTC-induced by Dibutyltin Dichloride joint Ethanol drinking at the indicated time. A: Amylase activity (U/L); B: Level of bilirubin (mmol/L); C: Concentration of hyaluronic acid (μg/L). Values are shown as mean ± SD (n = 8). Results are expressed as mean ± SD compared with control; ^aP < 0.05 vs control.

Figure 3 Immunohistochemistry of macrophages and T lymphocytes in DBTC-induced pancreatitis at different time-point. A: Macrophages were stained using an anti-mice macrophage antibody (F4/80) (original magnification: 400 ×); B: T lymphocytes were stained using an anti-mice CD3 monoclonal antibody (original magnification: 400 ×).

Figure 4 Fibrotic parameter changes in pancreas of mice with chronic pancreatitis induced DBTC-induced by dibutyltin dichloride joint ethanol drinking at the indicated time. A: FN expression in pancreas with immunofluorescence double staining of FN and DAPI showing co-expression in the pancreas (original magnification: 200 ×); B: COL1A1 expression in pancreas with western blot.

Figure 5 MMP-1mRNA and TIMP-1mRNA changes in pancreas of mice with chronic pancreatitis induced DBTC-induced by dibutyltin dichloride joint ethanol drinking at the indicated time. A: MMP-1 mRNA expression in pancreas with Q-PCR; B: TIMP-1 mRNA expression in pancreas with Q-PCR. Values are shown as mean ± SD (n = 8). Results are expressed as mean ± SD. ^aP < 0.05 vs control.