Crude extract of maggots: Antibacterial effects against Escherichia coli, underlying mechanisms, separation and purification

Figure 1 Antibacterial effects of different concentrations of the crude extract of maggots.

Figure 2 Determination of the effects of different concentrations of the crude extract of maggots on bacterial membrane permeability by fluorescence probe technique. Antibiotic, crude extract, unit: mg/mL; bacteria: 1 × 10⁸ CFU/mL.

Figure 3 Determination of the effects of different concentrations of the crude extract of maggots on plasmid DNA replication by agarose gel electrophoresis. The left-most lane: DNA marker; 1: Marker; 2: Crude extract 2 mg/mL + plasmid; 3: Crude extract 80 mg/mL + plasmid; 4: TNE + plasmid; 5: Plasmid alone; 6: Gentamicin 50 mg/mL + plasmid; 7: Gentamicin 100 mg/mL + plasmid. Antibiotic, crude extract; unit: mg/mL.

Figure 4 Antibacterial effects of the crude extract of maggots, eluent and eluant obtained from DEAE-Sepharose ion exchange chromatography. A: SDS-PAGE analysis of the crude extract of maggots, eluent and eluant. 1: Marker; 2: Crude extract 1 mg/mL; 3: Crude extract 0.5 mg/mL; 4: Eluent 1 mg/mL; 5: Eluent; 0.5 mg/mL; 6: Eluant 1 mg/mL; the left-most lane: marker (unit: kDa); B: Antibacterial effects of the crude extract of maggots 1 mg/mL, eluent and eluant.

Figure 5 Antibacterial effects of filtrates obtained from sephacryls-200HR gel filtration chromatography. A: SDS-PAGE analysis of filtrates from Sephacryls-200HR gel filtration chromatography. 1: Marker (kDa); 2: tube 12; 3: tube 11; 4: tube 10; 5: tube 9; 6: tube 8; 7: tube 7; 8: tube 6; 9: tube 5; 10: tube 4; B: Antibacterial effects of filtrates from sephacryls-200HR gel filtration chromatography. OD: Optical density.