Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Feb 7, 2015; 21(5): 1479-1487
Published online Feb 7, 2015. doi: 10.3748/wjg.v21.i5.1479
Figure 1
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Figure 1 Effects of Xiaotan Sanjie decoction and interleukin-8 on invasion of SGC-7901 cells. The number of invasive cells increased following addition of IL-8. In contrast, this number was significantly decreased by XTSJ decoction-containing serum. Importantly, XTSJ decoction decreased the number of invasive cells when SGC-7901 cells were exposed to IL-8. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.
Figure 2
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Figure 2 Effects of interleukin-8 and Xiaotan Sanjie decoction on SGC-7901 cell migration (scratch wound assay). Monolayers of cells treated with various interventions were wounded with pipette tips. The images obtained after 12 h and 24 h demonstrate that IL-8 enhanced the migration capability; in contrast, XTSJ decoction reduced this capability. Importantly, the scratched area recovered more slowly in the IL-8 + XTSJ group than in the IL-8 group. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.
Figure 3
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Figure 3 Effects of Xiaotan Sanjie decoction and interleukin-8 on CD44 protein expression in SGC-7901 cells (immunofluorescence staining). CD44 immunostaining was restricted to the cell membrane. Nuclei are marked with DAPI (blue). CD44 is counterstained with Cy3 (Red). XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.
Figure 4
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Figure 4 Effects of Xiaotan Sanjie decoction and interleukin-8 on CD44 protein expression in SGC-7901 cells (Western blot). CD44 was significantly up-regulated by IL-8, but down-regulated by XTSJ decoction. No interaction was found between XTSJ decoction and IL-8. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.
Figure 5
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Figure 5 Effects of Xiaotan Sanjie decoction and interleukin-8 on the expression of CD44 mRNA in SGC-7901 cells (quantitative PCR). A: Marker DL2000 (from top to bottom: 1000, 750, 500, 250, 100 bp); B-E: hGAPDH (blank group, IL-8 group, XTSJ group, XTSJ + IL-8 group, 145 bp); F-I: CD44 (blank group, IL-8 group, XTSJ group, XTSJ + IL-8 group, 230 bp). CD44 mRNA expression was determined by quantitative PCR. Similar to protein expression, CD44 mRNA expression was significantly up-regulated by IL-8, but was not influenced by XTSJ decoction. Importantly, XTSJ decoction down-regulated the CD44 mRNA expression induced by IL-8. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.