Hepatic artery bridging lessens temporary ischemic injury to bile canaliculi

Figure 1 Schematic diagram of animal model building.

Figure 2 Serum chemistry and hepatic cord structure are altered after surgery. A: Serum ALT levels; B: Serum TB levels. C: Serum ALP levels; D: Serum γ-GT levels; E: HE staining at 1 mo (magnification × 400). Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group. Group A vs Group D, ^cP < 0.05; Group B vs Group D, ^eP < 0.05; Group B vs Group C, ^gP < 0.05.

Figure 3 Peak velocity and blood flow in the hepatic artery in group D. A: Peak velocity, P > 0.05; B: Blood flow, P > 0.05. Group D: Hepatic artery bridging group. Pre-HAB: Pre-hepatic artery bridging; HAB: Hepatic artery bridging.

Figure 4 Morphological changes in bile canaliculi as visualized by transmission electron microscopy (magnification × 40000). Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group.

Figure 5 Apoptotic index (percentage of apoptotic cells in the total number of hepatocytes) for all groups. Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group. ^aP < 0.05, ^cP < 0.05 vs Group B.

Figure 6 Comparison of the density values per area of cofilin and HSP27 in the liver. A: Cofilin; B: HSP27; C: Immunohistochemical staining at 1 mo. Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group. ^aP < 0.05, ^cP < 0.05 vs Group B.

Figure 7 Expression of cofilin and HSP27 for all groups. A: Cofilin; B: HSP27; C: Western blots of cofilin and HSP27. Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group. ^aP < 0.05, ^cP < 0.05 vs Group B.

Figure 8 The cofilin, HSP27 and F-actin mRNA levels as determined by real-time polymerase chain reaction. A: Cofilin; B: HSP27; C: F-actin. Group A: Control group; Group B: Hepatic artery ischemia group (1 h); Group C: Hepatic artery ischemia group (2 h); Group D: Hepatic artery bridging group. ^aP < 0.05, ^cP < 0.05 vs Group B.