Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Aug 21, 2015; 21(31): 9358-9366
Published online Aug 21, 2015. doi: 10.3748/wjg.v21.i31.9358
Figure 1
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Figure 1 Transmission electron microscopy of cathartic colons. In the colons of control animals, normal A: Nerve fibers (Nf; magnification × 40000); and B: Interstitial cells of Cajal (ICC; magnification × 2500) can be seen. In contrast, cathartic colons in model animals showed C: Vacuole formation in ‘mit’ and sparse neurofilament (magnification × 11500); D: Eosinophil (EOS) infiltration (magnification × 1750); E (magnification × 5900) and F (magnification × 5900) each for mosapride and FAI showing recovery of damage.
Figure 2
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Figure 2 Relative mRNA expression of 5-hydroxytryptamine receptor 4 and neurofilament-H in rat colon tissue. aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs model. FAI: Fructus aurantii immaturus; H: High dose (4 g/kg); L: Low dose (1 g/kg); M: Medium dose (2 g/kg); NF-H: Neurofilament-H; 5-HTR4: 5-hydroxytryptamine receptor 4.
Figure 3
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Figure 3 Protein expression of 5-hydroxytryptamine receptor 4 and neurofilament-H in rat colon tissue. A: Representative Western blots showing 5-HTR4 and NF-H protein expression in rat colon tissue: 1 = normal, 2 = model, 3 = mosapride, 4 = FAI-L, 5 = FAI-M, 6 = FAI-H; B: Quantification of protein expression. aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs model. FAI: Fructus aurantii immaturus; H: High dose (4 g/kg); L: Low dose (1 g/kg); M: Medium dose (2 g/kg); NF-H: Neurofilament-H; 5-HTR4: 5-hydroxytryptamine receptor 4.
Figure 4
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Figure 4 Immunohistochemical staining for 5-hydroxytryptamine receptor 4 in rat colon tissue. A: Control group; B: Model group; C: Mosapride group; D: Low dose (1 g/kg) aqueous fructus aurantii immaturus (FAI) group; E: Medium dose (2 g/kg) aqueous FAI group; F: High dose (4 g/kg) aqueous FAI group (magnification × 200).
Figure 5
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Figure 5 Immunohistochemical staining for neurofilament-H in rat colon tissue. A: Control group; B: Model group; C: Mosapride group; D: Low dose (1 g/kg) aqueous fructus aurantii immaturus (FAI) group; E: Medium dose (2 g/kg) aqueous FAI group; F: High dose (4 g/kg) aqueous FAI group (magnification × 200).