Opioid growth factor and the treatment of human pancreatic cancer: A review

Figure 1 Opioid growth factor receptor is required for the inhibitory action of opioid growth factor and the stimulatory action of NTX on growth of MIA PaCa-2 human pancreatic cell cultures. Cells were transfected with opioid growth factor receptor (OGFr) siRNA or scrambled siRNA for 24 h and then treated with 10^-6 mol/L. Opioid growth factor (OGF) or NTX, or 100 μL of sterile water for 72 h; compounds and media were replaced daily. Values are expressed as mean ± SE for cell counts from 2 aliquots/well and at least 2 wells/treatment. ^bP < 0.001 vs untransfected cultures.

Figure 2 Tumor growth of MIA PaCa-2 tumors xenografted into nude mice. Animals were injected with 10 mg/kg opioid growth factor (OGF) daily, 120 mg/kg gemcitabine every 3 d (gemzar), both OGF and Gemzar, or 0.1 mL of sterile saline daily (control). Tumor volumes were monitored with calipers over a 45-d period of time. Values represent mean ± SE for all mice in the group. See original manuscript[39] for statistical comparisons.