Investigation of cholecystokinin receptors in the human lower esophageal sphincter

Figure 1 Arrangement of the sling and clasp muscles in the human gastroesophageal junction viewed from the distal luminal aspect after resection of the submucosa.

Figure 2 Western blots of the cholecystokinin-A and cholecystokinin-B receptors in the human sling and clasp muscles. CCK: Cholecystokinin.

Figure 3 Representative saturation isotherms of the binding of ³H-cholecystokinin-8S to membrane proteins isolated from the human sling (A) and clasp (B) fibers. CCK: Cholecystokinin.

Figure 4 Representative competitive inhibition curves of the binding of ³H- cholecystokinin-8S to membrane proteins isolated from the human sling (A) and clasp (B) fibers. The antagonists used were CR-1409 [selective for cholecystokinin (CCK)-A receptors] and CR-2945 (CCK-B receptors). Binding is expressed as the percentage of radioactivity specifically bound in the absence of antagonists.