Simple animal model of Helicobacter pylori infection

Figure 1 Illustration of Helicobacter pylori rat inoculation. A: Male Sprague-Dawley rats 120-150 g; B: Pretreatment with streptomycin (5 mg/kg) for three consecutive days; C and D: H. pylori in microaerophilic condition: 5% O₂, 50% CO₂, 37 °C; E and F: H. pylori 10⁸-10¹⁰ CFU/mL, suspended in saline; G: Gavage, 1 mL/rat twice daily at an interval of 4 h, for three consecutive days. H. pylori: Helicobacter pylori.

Figure 2 Antral mucosa from Helicobacter pylori-infected rats with hematoxylin and eosin staining. A: Helicobacter pylori organism in the gastric mucosa (arrow) (600 ×); B: Gastric mucosa with erosion and scattered infiltration of inflammatory cells (250 ×).

Figure 3 Bar graph of the mean ± SE of adherent leukocytes and macromolecular leakage of control group compared with Helicobacter pylori-infected group. The leukocyte adhesion and macromolecular leakage in the H. pylori-infected group were significantly increased compared with the control group (^aP < 0.01 vs control group). H. pylori: Helicobacter pylori.

Figure 4 Intravital microscopy demonstrated leukocyte adhesion in control group (A and C) and Helicobacter pylori-infected group (B and D) (40 ×).

Figure 5 Intravital fluorescent microscopic images (20 ×) demonstrate macromolecular leakage from vessels to the interstitial fluid at 0 and 30 min after injection of control group (A and B) and Helicobacter pylori-infected group (C and D). FITC-dx-250 injection (0 min) (A and C); same area at 30 min after FITC-dx-250 injection (B and D).