Squamous cell carcinoma after radiofrequency ablation for Barrett's dysplasia

Figure 1 Endoscopic, genetic and histological figures of the patient before and immediately after radiofrequency ablation for Barrett’s related high grade dysplasia. A: Sanger sequencing from columnar lined oesophagus taken in June 2010 (2) showing a mutation in CDKN2A c.286 G > A (p.V100M) (starred). A control sequence is also shown (1); B: Haematoxylin and eosin stain at × 10 magnification from a biopsy taken in June 2010. This demonstrates high grade dysplasia with nuclear pleiomorphism, increased nuclear: cytoplasmic ratio and disordered nuclei (arrow); C: Endoscopic view of the patient’s columnar-lined oesophagus (April 2009) showing classical salmon pink mucosa between 28cm and 36cm from the incisors; D: Endoscopic view of the patient’s re-epithelialized oesophagus (September 2010) after radiofrequency ablation.

Figure 2 Endoscopic, genetic and histological figures of the patient after radiofrequency ablation. A: Endoscopic view of a 4cm ulcerating mass between 35 to 39 cm; B: Example of positive p63 stain of the biopsies taken from the ulcerating mass (arrow); C: Haematoxylin and eosin stain of biopsies taken from the ulcer crater demonstrating invasive squamous cell cancer (arrow); D: Sanger sequencing of a mutation in TP53 c.817 C>T p. R273C (upper panel, starred) Wild type Sanger sequence of TP53 (lower panel).