HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays

Figure 1 Photomicrograph of immunohistochemistry (× 100) in a whole-tissue section. Asterisks indicate two round voids where the tissue microarray cores (inset) were extracted.

Figure 2 Dual-color in situ hybridization (× 1000), human epidermalgrowth factor receptor 2 amplification. Black dots: Human epidermalgrowth factor receptor 2 gene; Pink dots: Chromosome 17.

Figure 3 Comparison of positivity (3+) using the HercepTest (A), 4B5 (B) and SP3 (C) antibodies (× 200).

Figure 4 Representative image of the intratumoral heterogeneity of HER2 expression (× 100). Arrows indicate areas with strong continuous membranous staining (score 3+) and arrowheads indicate negative areas (score 0).