PRSS1_p.Leu81Met mutation results in autoimmune pancreatitis

Figure 1 p. L81M mutation of the protease serine 1 gene in autoimmune pancreatitis family. Hatched symbols: Patients with chronic pancreatitis; Striped symbols: Individuals with suspected chronic pancreatitis; Arrows: Subjects who were available for genetic analysis; Plus: Presence of heterozygous mutation; Asterisk: Index patient.

Figure 2 Sequencing of gene mutations from the patients with autoimmune pancreatitis. A: The sequencing c.247 C > A of PRSS1 gene mutation (p.81Leu→Met); B: Sequencing c.279 C > A of PRSS1 gene silent mutation (p.91Ala→Ala). The red arrow indicates the base mutation.

Figure 3 Histopathologic examination of the pancreas. A: A large number of lymphocytes and plasma cells were found in the bile ducts accompanied by hyperplasia of myofibroblasts (hematoxylin-eosin, × 20); B: The number of pancreatic acinars was markedly decreased (immunohistochemistry staining of cytokeratin, × 20).

Figure 4 Ratio of trypsin/amylase among the three groups.

Figure 5 Polycystic lesions in the liver, gallbladder, pancreas, and spleen, and retroperitoneal lymphadenopathy and bullae. A: Abdominal magnetic resonance imaging figure showed diffuse swelling; B: Computed tomography findings image of lung.