Effect of growth hormone, hyperbaric oxygen and combined therapy on the gastric serosa

Figure 1 Surgical procedure and histopathological assessment. A, B: Anastomotic line is shown between the gastric surface and jejunum, C: Outer surface of the neomucosa formation is shown with the jejunal segment; D: Inner surface of the neomucosa is shown, and the neomucosa has a typical small intestinal phenotype.

Figure 2 Histological and morphologic evaluation. A: Unexpected neomucosal formation. The gastric corpus mucosa can be seen. The squamous epithelium and lamellar keratinization formed from the anastomosis [Hematoxylin and eosin (HE) × 100] (Group 3); B: Granulation tissue and newly formed neomucosa. The blue area is connective tissue (Masson Trichrome × 100) (Group 4); C: In the gastric mucosa of the large granulation tissue, newly formed goblet cells can be seen (HE × 100) (Group 4); D: The left side shows the gastric mucosa, and the right side shows newly formed neomucosa that contains mucin. The granulation tissue is reduced (HE × 100) (Group 4).

Figure 3 There were significant differences in the villus density in all of the groups compared with the control group. A: Gastric corpus mucosa on the left, newly formed thin neomucosa on the right. The muscularispropria has not yet formed. The granulation tissue regressed [Hematoxylin and eosin (HE) × 100] (Group 3); B: Neomucosa formation is observed at the bottom of the gastric mucosa (HE × 125) (Group 4); C: The granulation tissue is in the middle, with newly formed neomucosa on either side. At the bottom, the stomach tissue is visible (HE x 100) (Group 2); D: The early development of the mucosal layer and granulation tissue (HE x 125) (Group 1).