Correlation of human epidermal growth factor receptor 2 expression with clinicopathological characteristics and prognosis in gastric cancer

Figure 1 Fluorescent in-situ hybridization analysis of human epidermal growth factor receptor 2 gene amplification (× 600). A: Normal human epidermal growth factor receptor 2 (HER2) gene expression: Red signals (HER2 gene), green signals [chromosome enumeration probe 17 (CEP17)], blue signals (nuclei lining dye); B: Positive HER2 gene amplification: HER2:CEP17 > 2; C: Positive HER2 gene amplification: HER2:CEP17 > 2 with clear red cluster signals observed.

Figure 2 Immunohistochemical analysis of human epidermal growth factor receptor 2 protein expression (× 200). A: Immunohistochemical (IHC) 0: No staining on tumor cell membrane; B: IHC1+: Faintly perceptible staining on > 10% tumor cell membrane; C: IHC2+: Moderate staining on > 10% tumor cell membrane; IHC3+: Strong staining on > 10% tumor cell membrane.

Figure 3 Kaplan-Meier survival analysis. A: Overall survival curves of 171 gastric cancer patients according to human epidermal growth factor receptor 2 (HER2) detection (P = 0.3386); B: Survival curve of patients with well differentiated gastric cancer according to HER2 expression (P = 0.0123); C: Survival curve of patients with poorly differentiated gastric cancer according to HER2 expression (P = 0.0988); D: Survival curve of patients with the diffuse/mixed type gastric cancer according to HER2 expression (P = 0.6623).