Novel biosensor-based microarray assay for detecting rs8099917 and rs12979860 genotypes

Figure 1 Designed interleukin 28B biosensor-based microarray and results of constructed plasmids detected by biosensor microarray assay. a: rs12979860C; b: rs12979860T; c: rs8099917 T; d: rs8099918 G; e: System control probes; f: Positive probes; g: Negative probes.

Figure 2 Polymerase chain reaction products visualized on polyacrylamide gel electrophoresis. Lanes 1 and 8: Marker; Lanes 2, 5, 6: A single fragment amplified rs12979860 or rs8099917 primers; Lanes 3 and 4: Two fragments amplified by one tube polymerase chain reaction system of rs12979860 and rs8099917; Lane 7: Negative control.

Figure 3 Patterns detected by biosensor microarray assay for rs8099917 and 12979860 genotypes.