Brief Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 7, 2012; 18(33): 4618-4626
Published online Sep 7, 2012. doi: 10.3748/wjg.v18.i33.4618
Figure 1
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Figure 1 Inhibition of cell viability by genistein and genistein analogues. A: AGS cell line; B: SGC-7901 cell line. aP < 0.05 vs treatment with genistein; cP < 0.05 vs treatment with genistein or other genistein analogues. 1: Genistein (5,7,4'-trihydroxylisoflavone); 2: 7-difluoromethyl genistein; 4a: 7-difluoromethyl-5,4'-dimethyl genistein; 4b: 7-difluoromethyl-5,4'-diethyl genistein; 4c: 7-difluoromethyl-5,4’-di-n-propyl genistein; 4d: 7-difluoromethyl-5,4’-di-benzyl genistein; 4e: 7-difluoromethyl-5,4'-diheptyl genistein; 4f: 7-difluoromethyl-5,4'-di-n-octyl genistein; 4g: 7-difluoromethyl-5,4'-didecyl genistein; 4h: 7-difluoromethyl-5,4'-diisobutyl genistein.
Figure 2
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Figure 2 Decrease of colony number and inhibition of colony formation by 7-difluoromethoxyl-5,4’-di-n-octylgenistein. A: Decrease of colony number by 7-difluoromethoxyl-5,4’-di-n-octylgenistein (DFOG); B: Inhibition of colony formation by DFOG and genistein in AGS cell line. aP < 0.05 vs treatment with dimethyl sulfoxide (DMSO); cP < 0.05 vs treatment with 10 μmol/L genistein (GEN) or 1 μmol/L DFOG.
Figure 3
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Figure 3 Increase of cells in G2/M phase and induction of cell cycle arrest in G2/M phase by 7-difluoromethoxyl-5,4’-di-n-octylgenistein. A: Increase of cells in G2/M phase by 7-difluoromethoxyl-5,4’-di-n-octylgenistein (DFOG); B: Induction of cell cycle arrest in G2/M phase by DFOG and genistein in AGS cell line. aP < 0.05 vs treatment with dimethyl sulfoxide (DMSO); cP < 0.05 vs treatment with 10 μmol/L genistein (GEN) or 1 μmol/L DFOG.
Figure 4
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Figure 4 Down-regulation of forkhead box M1 expression by 7-difluoromethoxyl-5,4’-di-n-octylgenistein and genistein in AGS and SGC-7901. A: mRNA level using reverse transcription-polymerase chain reaction in AGS cell line; B: Protein level using Western blotting in AGS cell line; C: Decrease of forkhead box M1 (FOXM1) protein expression in SGC-7901 cell line. DMSO: Dimethyl sulfoxide; GEN: Genistein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Figure 5
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Figure 5 Modulation of the protein expressions of forkhead box M1 downstream target genes by 7-difluoromethoxyl-5,4’-di-n-octylgenistein and genistein. A: AGS cell line; B: SGC-7901 cell line. DMSO: Dimethyl sulfoxide; GEN: Genistein; DFOG: 7-difluoromethoxyl-5,4’-di-n-octylgenistein.
Figure 6
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Figure 6 Forkhead box M1 small interfering RNA enhances the down-regulation of forkhead box M1 protein expression by 7-difluoromethoxyl-5,4’-di-n-octylgenistein and effects of forkhead box M1 small interfering RNA transfection or 7-difluoromethoxyl-5,4’-di-n-octylgenistein in AGS cell line. A: Western blotting analysis; B: The cell viability inhibitory effects of 7-difluoro methoxyl-5,4’-di-n-octylgenistein (DFOG) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte trazolium bromide assay; C: Both on cell cycle distribution in AGS cells. aP < 0.05 vs treatment with dimethyl sulfoxide (DMSO); cP < 0.05 vs treatment with 5 μmol/L DFOG or forkhead box M1 (FOXM1) small interfering RNA (siRNA) alone.
Figure 7
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Figure 7 Forkhead box M1 cDNA transfection reduces the down-regulation of forkhead box M1 protein expression by 7-difluoromethoxyl-5,4’-di-n-octylgenistein in AGS cell line. A: Western blotting analysis; B: The cell viability inhibitory effects of 7-difluoro methoxyl-5,4’-di-n-octylgenistein (DFOG) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte trazolium bromide assay. aP < 0.05 vs treatment with dimethyl sulfoxide (DMSO); cP < 0.05 vs treatment with 5 μmol/L DFOG or forkhead box M1 (FOXM1) cDNA transfection alone.