Screening Helicobacter pylori genes induced during infection of mouse stomachs

Figure 1 Construction of Helicobacter pylori specific antibiotic-based in vivo expression technology plasmids, pIVET11 and pIVET12. These plasmids are derivatives of plasmid pIVET8[10]. A: AatII; B: BamHI; Bg: BglII; bla: β-lactamase; kan: Kanamycin gene; mob: Plasmid mobilization; trpA: Tryptophan synthase α-subunit.

Figure 2 Helicobacter pylori specific in vivo expression technology strategy. H. pylori: Helicobacter pylori; B: BamHI; Bg: BglII; kan: Kanamycin gene.

Figure 3 Gene expression of Helicobacter pylori induced by phagocytosis. Up-regulation and down-regulation of Helicobacter pylori in vivo induced genes expressed by macrophage engulfed bacteria.