Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis

Figure 1 Matrix metalloproteinases-3, matrix metalloproteinases-7 and tissue inhibitor of matrix metalloproteinase-2 in lower (A, C, E1, respectively) and higher grade of inflammation and calprotectin levels (B1, D1, F, respectively), tissue inhibitor of matrix metalloproteinase-3 in pouch (G1). Black solid arrows plasma cells, black dotted arrows macrophages, red solid arrows eosinophils and red dotted arrows endothelium. Scale bars: 15 μm (A, B1, C, D1, E1, F, G1); 7.5 μm (B2-B4, D2, D3, E2, G2, G3). Stainings were performed using diaminobenzidine or NovaRED as chromogenic substrates and Mayer’s hematoxylin as counterstain. Images were obtained using a light-field microscope, and edited using Adobe Photoshop 7.0 (Adobe Systems Incorporated).

Figure 2 matrix metalloproteinases-8 (A1), matrix metalloproteinases-9 (D1, E1), matrix metalloproteinases-12 (F1, G), matrix metalloproteinases-26 (B1) and tissue inhibitor of matrix metalloproteinase-1 (C) in pouch. Inset g’ added to figure from another sample, not shown in here in lesser magnification. Arrowheads depict neutrophils, black solid arrows plasma cells, black dotted arrows macrophages, red solid arrows eosinophils. Scale bars: 15 μm (A1, B1, C, D1, E1, F1,G); 7.5 μm (A2, B2, B3, D2, D3, E2, F2, F3). Stainings were performed using diaminobenzidine or NovaRED as chromogenic substrates and Mayer’s hematoxylin as counterstain. Images were obtained using a light-field microscope, and edited using Adobe Photoshop 7.0 (Adobe Systems Incorporated).