Aberrant methylation and downregulation of sall3 in human hepatocellular carcinoma

Figure 1 Average methylation levels were calculated from 38 tumors (T) and adjacent non-cancerous tissues (N) on 24 CpG units from sall3 CpG island respectively. The data was analyzed by Wilcox rank sum test (^aP < 0.05 vs non-cancerous tissues ). Error bar, 95% confidence interval.

Figure 2 sall3 mRNA is analyzed in 38 tumor tissues (T) and adjacent non-cancerous tissues (N). Error bars, SD from triplicates.

Figure 3 Correlation between methylation levels and mRNA expression of sall3 in 24 tumor tissues and corresponding adjacent noncancerous tissues.

Figure 4 Quantitative methylation analysis on each CpG unit of sall3 CpG island in HCC cells after 5-Aza-CdR treatment or control (A-F).

Figure 5 Relative sall3 expression in six hepatocellular carcinoma cells after 5-AZA-CdR treatment. Error bars, SD from triplicates.