Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma

Figure 1 Detection of Rab27A and Rab27B mRNA and protein in 6 human cell lines. A and C: Reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR, respectively, of Rab27A in 5 hepatocellular carcinoma cell lines and the human HL-7702 line; D and F: RT-PCR and real-time PCR, respectively, of Rab27B; B and E: Western blotting of Rab27A and Rab27B protein levels, respectively. β-actin served as an internal control.

Figure 2 Differential expression of Rab27A and Rab27B in hepatocellular carcinoma patients. A: Positive staining of Rab27A in hepatocellular carcinoma (HCC) tissue; B: Negative staining of Rab27A in adjacent tissue; C: Negative staining of Rab27B in HCC tissue; D: Positive staining of Rab27B in adjacent tissue; E and F: Positive staining of Rab27A and Rab27B, respectively, in the same HCC tissue. Both Rab27A and Rab27B were observed in the cytoplasm and plasma membrane of human cells. Original magnification × 100; the inset boxes are at original magnification × 400.

Figure 3 Kaplan-Meier overall survival analysis of Rab27A and Rab27B in hepatocellular carcinoma patients. A: Survival analysis of hepatocellular carcinoma patients by expression of Rab27A; B: Survival analysis by expression of Rab27B; C: Survival analysis with expression of Rab27A and Rab27B as covariables.