Effects of CPG ODN on biological behavior of PANC-1 and expression of TLR9 in pancreatic cancer

Figure 1 Western blotting test for toll-like receptor 9 protein. 1: Normal human pancreatic tissue; 2: Human peritumoral tissue; 3: Human pancreatic cancer tissue. TLR-9: Toll-like receptor 9; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Figure 2 Expression of toll-like receptor 9 protein. Brown color displays the positive expression. A: Human pancreatic cancer tissue (× 200); B: Peritumoral human pancreatic cancer tissue (× 200); C: Normal pancreas tissue (× 400).

Figure 3 Immunofluorescence staining test. The expression of toll-like receptor 9 (TLR9) was mainly detected in Panc-1 cell cytoplasm (× 200). A: TLR9 was highly expressed in Panc-1 cells; B: TLR9 was negatively expressed in control group.

Figure 4 Inhibitory effect of cytosine phosphate-guanosine oligodeoxynucleotides 2216 of different concentrations on Panc-1 cell line with growth inhibition rate increasing remarkablly with concentration and time.

Figure 5 Panc-1 cell scratch test at various time points (× 400). Compared with control group, a higher cytosine phosphate-guanosine oligodeoxynucleotides 2216 concentration led to significantly lower migration ability (P < 0.01).

Figure 6 Invasion ability of Panc-1 in cabinet (× 200). A: In control group, there are more Panc-1 cells penetrating through filter membrane; B: The cell number of 1 mg/mL cytosine phosphate-guanosine oligodeoxynucleotides (CPG ODN) treated group penetrating through filter membrane decreased significantly (A vs C, P < 0.01); C: The Panc-1 cell number of 10 mg/L CPG ODN treated group penetrating through filter membrane further decreased (A vs B, P <0.01).

Figure 7 Cell cloning test. A: More colonies are formed in the control group; B: The colony formed in the 1 mg/L cytosine phosphate-guanosine oligodeoxynucleotides (CPG ODN) treated group is the same as in the control group (P > 0.05); C: There are fewer colonies formed in the 10 mg/L CPG ODN treated group (A vs B, P < 0.01).