miR-200 family expression is downregulated upon neoplastic progression of Barrett’s esophagus

Figure 1 Top associated network functions for miR-141 and miR-200c. A: Ingenuity Pathway Analysis predicted that the top associated network functions for miR-141 were gene expression, cell death and cell cycle (P = 1 × 10^-35). B: The top associated network functions for miR-200c were cell morphology, cellular assembly and organization, cellular function and maintenance (P = 1 × 10^-38). Predicted targets of miR-141 or miR-200c are highlighted in grey. Uncolored entries represent molecules that are associated with the pathway but are not predicted miR-141 or miR-200c targets. The P values were derived from a right-tailed Fisher’s exact test to calculate the probability that each predicted miRNA target matches the ascribed network function due to chance alone.

Figure 2 miR-200 family expression in benign Barrett’s and dysplastic cell lines. Relative expression of all miR-141, miR-200a, miR-200b and miR-429, normalized to U44 expression is shown on the left hand y axis. Relative expression of miR-200c is shown on the right hand y axis. The pattern of relative expression of miR-200 members in the Qh (benign Barrett’s) cell line closely resembled that in benign Barrett’s esophagus mucosa (see relative expression values in Table 1).