Upregulated CD133 expression in tumorigenesis of colon cancer cells

Figure 1 Fluorescence-activated cell sorting showing CD133 expression in different colorectal cancer cell lines (A), CD133 staining of human primary colorectal cancer tissue (B) and metastatic colorectal cancer tissue (C) (Original magnification × 100). Brown indicates positive staining.

Figure 2 Fluorescence-activated cell sorting showing CD133 expression in SW620, CD133^- and CD133^hi cells (A) and in their spheroids (B), CD133 staining in spheroids of SW620, CD133^- and CD133^hi cells (original magnification × 100, brown indicates positive staining) (C), and reverse transcription-polymerase chain reaction showing CD133 expression in SW620, CD133^- and CD133^hi cells and their spheroids. ^aP < 0.05 vs monolayer cells. SP: Spheroid.

Figure 3 Quantitative reverse transcription-polymerase chain reaction showing Lgr5 expression in SW620, CD133^- and CD133^hi cells and their spheroids. ^aP < 0.05 vs monolayer cells. SP: Spheroid.