miR-93 suppresses proliferation and colony formation of human colon cancer stem cells

Figure 1 Human colon cancer stem cells grow into clonally derived spheres in serum-free medium.

Figure 2 Expression levels of select miRNAs in SW1116csc as measured with quantitative reverse transcription polymerase chain reaction.

Figure 3 Growth curves of SW1116csc, SWcon cells, and SWt cells. mean ± SD are shown.

Figure 4 Colony formation after incubation of 100 separate cells for 14 d. mean ± SD are shown.

Figure 5 Expression of HDAC8 and TLE4 mRNA and protein in SW1116csc, SW1116 cells and SWt cells. 1: SW1116csc; 2: SW1116 cells; 3: SWt cells. Glyceraldehyde-3-phosphate dehydrogenase was evaluated as an internal control. RT-PCR: Reverse transcription polymerase chain reaction; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.