Integration of human papillomavirus 18 DNA in esophageal carcinoma 109 cells

Figure 1 Detection of HPV18 DNA in EC109 cells. A-D: HPV18 DNA detection in EC109 cells using the primer pairs Y09/11, GP5 +/6 +, HPV18E6, and HPV18E6-E7, respectively. M1: 100 bp DNA ladder; Neg: Negative control (template of HEK 293 cell DNA); HeLa: Positive control (template of HPV18-infected cervical cancer cells; EC109: DNA from EC109 cells. HPV: Human papillomavirus; EC: Esophageal carcinoma.

Figure 2 Alignment sequencing results for esophageal carcinoma109 cells with human papillomavirus18. Query 288-388, sequencing results for esophageal carcinoma109 cells after polymerase chain reaction amplification with P2-18E7-specific primers; Sbjct 844-944, partial sequence of human papillomavirus18E7-E1 from X05015 in GenBank.

Figure 3 Alignment sequencing results for esophageal carcinoma109 cells with human chromosome. Query 227-483, sequencing results for esophageal carcinoma109 cells after polymerase chain reaction amplification with P2-18E7-specific primers; Sbjct 41562135-41562391, partial sequence of human chromosome 8.