Brief Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastroenterol. May 21, 2011; 17(19): 2437-2445
Published online May 21, 2011. doi: 10.3748/wjg.v17.i19.2437
Figure 1
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Figure 1 Experimental design and procedure. OVA: Ovalbumin; BSA: Bovine serum albumin; DTH: Delayed-type hypersensitivity; DSS: Dextran sulfate sodium; qod: Every other day; sc: Subcutaneous injection; CEP: Colonextracted.protein
Figure 2
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Figure 2 Delayed-type hypersensitivity was scored at days 7, 14 and 21 after ovalbumin injection in appendectomized and sham-operated mice. aP < 0.05 vs bovine serum albumin (BSA) control mice in appendectomized or sham-operated groups. OVA: Ovalbumin.
Figure 3
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Figure 3 Delayed-type hypersensitivity on days 1 and 7 with 5% dextran sulfate sodium treatment after ovalbumin- or bovine serum albumin-ingestion and ovalbumin-injection in appendectomized and sham-operated mice. aP < 0.05 vs bovine serum albumin (BSA) control mice in sham-operated group. DSS: Dextran sulfate sodium; OVA: Ovalbumin.
Figure 4
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Figure 4 Histological findings in colon-extracted protein- or bovine serum albumin-treated mice in appendectomized (A and B) and sham-operated (C and D) groups. In the appendectomy group, there were similar histological features, massive lymphocyte infiltration, loss of goblet cells, crypt damage, mucosal ulceration, and accompanying submucosal edema, whereas in sham-operated mice, there was less amelioration of colonic inflammation. CEP: Colon-extracted protein; BSA: Bovine serum albumin.
Figure 5
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Figure 5 Blinded histological assessment of colitis at day 7 after treatment with 5% dextran sulfate sodium in appendectomized and sham-operated mice with colon-extracted protein or bovine serum albumin ingestion, based on the validated scoring system. aP < 0.05 vs bovine serum albumin (BSA)-treated mice in sham-operated group. CEP: Colon-extracted protein.
Figure 6
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Figure 6 Th1, Th2 and the ratio of Th1/Th2 in spleen lymphocytes at day 7 after treatment with 5% dextran sulfate sodium in appendectomized and sham-operated mice with colon-extracted protein or bovine serum albumin ingestion. CEP: Colon-extracted protein; BSA: Bovine serum albumin.
Figure 7
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Figure 7 Th1, Th2 and the ratio of Th1/Th2 in Peyer’s patch lymphocytes at day 7 after treatment with 5% dextran sulfate sodium in appendectomized and sham-operated mice with colon-extracted protein or bovine serum albumin ingestion. aP < 0.05 vs bovine serum albumin (BSA)-treated mice in the sham-operated group. CEP: Colon-extracted protein.