p73 G4C14 to A4T14 polymorphism is associated with colorectal cancer risk and survival

Figure 1 Detection of the p73 genotype by polymerase chain reaction with confronting two-pair primers. Lane M: 100-bp DNA ladder; Lanes 1 and 5: GC/GC genotype; Lanes 2, 3 and 6: GC/AT genotype; Lane 4: AT/AT genotype.

Figure 2 GC/GC genotype (A), GC/AT genotype (B), AT/AT genotype (C) confirmed by sequencing.

Figure 3 Immunohistochemical analysis of p73 in colorectal cancer tissues (immunohistochemistry, × 200). A: An adenocarcinoma with the GC/GC genotype revealed positive nuclear immunoreactivity for p73; B: An adenocarcinoma with AT/AT genotype revealed negative immunoreactivity for p73.

Figure 4 The p73 G4C14 to A4T14 polymorphism and colorectal cancer survival. Kaplan-Meier analysis of overall survival for 383 colorectal cancer patients shows that the patients with the GC/GC genotype are associated with worse survival compared with those with other genotypes (P = 0.02).