α,β-amyrin, a natural triterpenoid ameliorates L-arginine-induced acute pancreatitis in rats

Figure 1 Effects of α,β-amyrin treatment on serum amylase (A), lipase (B) and on pancreatic edema (C) in rats on L-arginine-induced acute pancreatitis. Each column represents mean ± SE (n = 8). ^aP < 0.05 vs saline control group; ^cP < 0.05 vs vehicle control group. Pred: Methylprednisolone.

Figure 2 Effect of α,β-amyrin treatment on the serum tumor necrosis factor α (A) and interleukin-6 (B) in L-arginine induced acute pancreatitis. Each column represents mean ± SE (n = 8). ^aP < 0.05 vs saline control group; ^cP < 0.05 vs vehicle control group. Pred: Methylprednisolone; TNF-α: Tumor necrosis factor α; IL-6: Interleukin-6.

Figure 3 Effects of α,β-amyrin treatment on the pancreatic myeloperoxidase activity (A), thiobarbituric acid-reactant substances (B) and nitrate/nitrite levels (C) in L-arginine induced acute pancreatitis. Each column represents mean ± SE (n = 8). ^aP < 0.05 vs saline control group; ^cP < 0.05 vs vehicle control group. Pred: Methylprednisolone; MPO: Myeloperoxidase; TBARS: Thiobarbituric acid-reactant substances.

Figure 4 Effect of α,β-amyrin on tumor necrosis factor α immunoreactivity in L-arginine-induced acute pancreatitis (× 400). A: Normal control group; B: Vehicle + L-arginine; C: α,β-amyrin (100 mg/kg) + L-arginine; D: Methylprednisolone (30 mg/kg) + L-arginine.

Figure 5 Effect of α,β-amirin on inducible nitric oxide synthetase immunoreactivity in L-arginine-induced acute pancreatitis (× 400). A: Normal control group; B: Vehicle + L-arginine; C: α,β-amyrin (100 mg/kg) + L-arginine; D: Methylprednisolone (30 mg/kg) + L-arginine.

Figure 6 Representatives microphotographs of pancreatic sections (× 400). A: Normal control group; B: Vehicle + L-arginine; C: α,β-amyrin (100 mg/kg) + L-arginine; D: Methylprednisolone (30 mg/kg) + L-arginine.