Brief Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Jun 28, 2010; 16(24): 3078-3082
Published online Jun 28, 2010. doi: 10.3748/wjg.v16.i24.3078
Figure 1
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Figure 1 Characterization of polymerase chain reaction (PCR) amplification by electrophoresis. A: Amplification of calreticulin (CRT)/hepatitis B surface antigen (HBsAg) fusion gene by PCR. Lane 1: Negative control; Lane 2: CRT/HBsAg fusion gene recombinant pJW4303 plasmid; Lane M: DNA marker; B: Characterization of CRT/HBsAg fusion gene recombinant pENTR/D-TOPO transfer vector by PCR. Lane 1: Negative control; Lane 2: CRT/HBsAg fusion gene recombinant pENTR/D-TOPO transfer vector amplified by specific PCR; Lane M: DNA marker.
Figure 2
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Figure 2 Culture of recombinant adenovirus in HEK293A cells. A: CPE was observed in recombinant adenovirus-containing HEK293A cells; B: Normal HEK293A cells cultured as control.
Figure 3
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Figure 3 Characterization of CRT/HBsAg fusion protein expression by Western blotting. The expression of CRT/HBsAg fusion protein in 293A cells was determined in 293A cells transfected with Ad-CRT/HBsAg and Ad-LacZ by Western blotting analysis. Human anti-HBsAg positive serum was used at a 1:100 dilution for the detection of CRT/HBsAg fusion protein expression. Lane 1: Lysate from 293 cells transfected with Ad-LacZ; Lane 2: Lysate from 293 cells transfected with Ad-CRT/HBsAg.