Aberrant expression of nuclear matrix proteins during HMBA-induced differentiation of gastric cancer cells

Figure 1 Two-dimensional protein profiles from the nuclear matrix of BGC-823 cells. A: Representative two-dimensional protein profiles from the nuclear matrix of BGC-823 cells before and after hexamethylene bisacetamide (HMBA) treatment. The differentially expressed proteins are marked with circular symbols on the gels. L1-L7 spots indicate the up-regulated proteins while L8-L15 indicates the down-regulated proteins; B: Enlarged portions from 2-DE gels.

Figure 2 Determination of prohibitin (PHB), nucleophosmin (NPM) and hnRNP A2/B1 at nuclear matrix proteins and whole cell proteins by Western immunoblotting. All of these three protein signals were found to be down-regulated at whole cell level and nuclear matrix level after HMBA treatment.

Figure 3 Effects of HMBA treatment on the localization of differentially expressed nuclear matrix proteins in NM-IF system as shown by a quantum dots-based immnuofluorescence. Typical changes of localization of NPM (A, B), PHB (C, D) and hnRNP A2/B1 (E, F) are observed after treatment with 5 mmol/L HMBA. The figure is composed of representative pictures taken from three independent experiments. A, C, E: Control; B, D, F: HMBA.