Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

Figure 1 Inhibitory effects of genistein on MHCC97-H cell proliferation. MHCC97-H cells were treated with various concentrations of genistein for 6 d, control cells were left untreated, A₅₉₅ values were determined daily.

Figure 2 Inhibitory effects of genistein on adhesion and invasion of MHCC97-H cells. A: MHCC97-H cells were incubated for 20, 40, 60 and 90 min with various concentrations of genistein; B: MHCC97-H cells were incubated for 20 h with various concentrations of genistein.

Figure 3 Western blotting analysis of protein expression and phosphorylation of FAK in MHCC97-H cells following genistein treatment. Lane 1: Genistein 5 μg/mL; Lane 2: Genistein 10 μg/mL; Lane 3: Genistein 20 μg/mL.

Figure 4 Effects of genistein on protein expression and phosphorylation of FAK. Black columns, protein expression of FAK; Open columns, phosphorylation of FAK. Values are mean ± SD from experiments.

Figure 5 Autopsy and microphotographic appearance after orthotopic implantation. A: Autopsy of mice bearing orthotopic implantations. Rapid tumor growth occurred. Thirty-five days after tumor tissue implantation into the liver; B: Microphotography of lung metastasis by MHCC97-H cells. Thirty-five days after orthotopic implantation, MHCC97-H cells metastasized to the lungs, forming pulmonary metastatic lesions. HE, × 200.