Measuring Ca2+ influxes of TRPC1-dependent Ca2+ channels in HL-7702 cells with Non-invasive Micro-test Technique

Figure 1 The Ca²⁺ fluxes of HL-7702 cells. A: A screen-printed picture of a cell measured by NMT; B: Net Ca²⁺ fluxes of a HL-7702 cell. The maximum values were 28.2 ± 8.2 pmol/cm² per second (n = 6); C: Net Ca²⁺ fluxes of a noradrenalin-treated HL-7702 cell. The maximum values were 71.7 ± 17.5 pmol/cm² per second (n = 6); D: Net Ca²⁺ fluxes of a nifedipine-treated HL-7702 cell. The maximum values were 29.0 ± 11.3 pmol/cm² per second (n = 6); E: Net Ca²⁺ fluxes of a La³⁺ -treated HL-7702 cell. The maximum values were 9.0 ± 3.5 pmol/cm² per second (n = 6); F: Bar graph of the maximum net Ca²⁺ influxes in four groups. ^bP < 0.01, control group vs noradrenalin-treated or La³⁺-treated group.

Figure 2 TRPC1-transfection influences Ca²⁺ influxes of HL-7702 cells. A: Net Ca²⁺ fluxes of a TRPC1-transfected cell. The maximum values were 48.9 ±

Figure 3 Nifedipine and La³⁺ influence Ca²⁺ influxes of TRPC1-transfected HL-7702 cells. A: Net Ca²⁺ fluxes of a TRPC1 + nifedipine-treated cell. The maximum values were 44.0 ± 5.7 pmol/cm² per second (n = 6); B: Net Ca²⁺ fluxes of a TRPC1 + La³⁺-treated cell. The maximum values were 7.6 ± 1.9 pmol/cm² per second (n = 6); C: Bar graph of the maximum net Ca²⁺ fluxes in the TRPC1-transfected group, TRPC1 + nifedipine-treated group, and TRPC1 + La³⁺-treated group. A significant statistical difference only existed between the TRPC1-transfected group and the TRPC1 + La³⁺-treated group (^bP < 0.01).