Comparative identification of Ca2+ channel expression in INS-1 and rat pancreatic β cells

Figure 1 The Ca²⁺ channel subunits expression profile in INS-1 and rat β cells. A: 12 of 15 Ca²⁺ channel subunits in rat pancreatic β cells. 1-5: Separately represent subunits TRPC1, TRPC4α, TRPC4β, TRPM2, and L-α1C; 6-10: Separately represent subunits L-α1D, L-α-S, L-α1F, IP₃R1, and IP₃R2; 11-15: IP₃R3, R-α1E, Ryr1, Ryr2, and N-α1B; M: 100 bp ladder marker, (bottom band, 100 bp; the expression profile of INS-1 is the same as for rat β cells, not shown); B: L-α-S, L-α1F, and Ryr1 expressed in rat heart and skeletal muscle. 1-3: L-α-S (cDNA of skeletal muscle), L-α1F (heart), and Ryr1 (cDNA of skeletal muscle); M: 100 bp ladder marker; bottom band of M, 100 bp.

Figure 2 Expression level of 12 Ca²⁺ channel subunits in INS-1 and rat primary β cells.