Orignal Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. May 14, 2009; 15(18): 2234-2239
Published online May 14, 2009. doi: 10.3748/wjg.15.2234
Figure 1
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Figure 1 ADFMChR selectively inhibited proliferation of HepG2 cells. aP < 0.05 vs treatment with ADFMChR in the same concentration to L-02 cells (mean ± SD, n = 9).
Figure 2
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Figure 2 Induction of apoptosis of HepG2 cells by ADFMChR. A: Treated with 0.2% DMSO; B: Treated with 30.0 &mgr;mol/L 5-FU; C: Treated with 30.0 &mgr;mol/L ChR; D: Treated with 3.0 &mgr;mol/L ADFMChR; E: Treated with 10.0 &mgr;mol/L ADFMChR; F: Treated with 30.0 &mgr;mol/L ADFMChR; G: Quantification of induction of apoptosis analysis of HepG2 cells. aP < 0.05 vs treatment with DMSO (mean ± SD, n = 3).
Figure 3
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Figure 3 DNA ladder assay showing ADFMChR-induced apoptosis of HepG2 cells. Lane 1: DNA marker; lane 2: Control; lane 3: 10.0 &mgr;mol/L ADFMChR (24 h); lane 4: 10.0 &mgr;mol/L ADFMChR + GW9662 (24 h); lane 5: 10.0 &mgr;mol/L ADFMChR (48 h); lane 6: 10.0 &mgr;mol/L ADFMChR + GW9662 (48 h); lane 7: 10.0 &mgr;mol/L ADFMChR (72 h); lane 8: 10.0 &mgr;mol/L ADFMChR + GW9662 (72 h).
Figure 4
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Figure 4 Western blotting analysis showing regulation of PPARγ (A), NF-κB (B), Bcl-2 (C) and Bax (D) protein expression in HepG2 cells by ADFMChR. (mean ± SD, n = 3).
Figure 5
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Figure 5 PPARγ antagonist GW9662 blocked the effects of ADFMChR on PPARγ and NF-κB protein expression in HepG2 cells. A: PPARγ; B: NF-κB. HepG2 cells were pretreated with 10.0 &mgr;mol/L GW9662 for 30 min, then exposed to 3.0, 30.0 &mgr;mol/L ADFMChR for 24 h, respectively (mean ± SD, n = 3).