Activator protein-1 involved in growth inhibition by RASSF1A gene in the human gastric carcinoma cell line SGC7901

Figure 1 The over-expression of RASSF1A in gastric cancer cell line SGC7901. Cells were stably transfected using Lipofectamine 2000 with RASSF1A or empty vector and were grown in RPMI containing G418 at 0.8 g/L. Resistant clones were selected separately and were measured by Western blotting and RT-PCR, β-actin was used as an internal loading control, and clone30 was used for further study. A: RASSF1A expression was analyzed by Western blotting; B: RASSF1A expression was analyzed by RT-PCR.

Figure 2 RASSF1A blocked gastric cancer cell line SGC7901 growth in vivo and in vitro. A: Cell growth curve of gastric cancer cell line SGC7901 measured by MTT assay; B: RASSF1A induced gastric cancer cell line SGC7901 G₁ arrest; C: RASSF1A inhibited the colony formation of SGC7901 cells measured by planting efficiency; D: RASSF1A inhibited the tumorigenicity of SGC7901 cells.

Figure 3 The over-expression of RASSF1A inhibited the AP-1 activity in gastric cancer cell line SGC7901. A: The over-expression of RASSF1A inhibited the AP-1 DNA binding activity measured by EMSA; B: The over-expression of RASSF1A inhibited the c-Fos but not c-Jun expression in nuclear extract of SGC7901 cells. (Western blotting, DAB staining); C: The over-expression of RASSF1A inhibited the c-Fos expression in nuclear of SGC7901 cells (immunocytochemistry, DAB staining, × 400); D: The over-expression of RASSF1A had no influence on the c-Jun expression in nuclear of SGC7901 cells (immunocytochemistry, DAB staining, × 400).