LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma

Figure 1 In situ hybridization analysis of GCRG123 in gastric mucosa. GCRG123 showed low expression (blue precipitates restricted to the cytoplasm) in intestinal-type adenocarcinoma (A) and normal gastric glands (C, left region); high expression in signet-ring cell carcinoma (B); normal epithelia (C); and normal pyloric glands (D). NBT/BCIP was used as an alkaline phosphatase substrate (magnification was 10 × 10).

Figure 2 Northern blotting of GCRG123 in gastric tissues. The expression of β-actin served as an internal control. Total RNA, 1 &mgr;g per lane, was separated on a 1% agarose/formaldehyde gel. After blotting onto a nylon membrane, the membrane was probed with digoxigenin-labeled anti-sense GCRG123 cRNA. N, normal gastric tissue; T, tumor; 1, signet-ring cell carcinoma; 2, intestinal-type adenocarcinoma.

Figure 3 Multalin analysis of GCRG123 (AF454554) and L1 (HUMTNL22). Red font represents high consensus, blue or black font represents low consensus.