Overexpression of the receptor tyrosine kinase EphA4 in human gastric cancers

Figure 1 RT-PCR analysis of the EphA4 and FGFR1 in gastric cancer cell lines.

Figure 2 Real time RT-PCR (A) and semiquantitative RT-PCR (B) analysis of the EphA4 in gastric cancer tissues. A: From left to right; case 1 tumor, case 2 tumor, case 3 tumor, case 4 tumor, case 3 normal, case 1 normal, and case 2 normal; B: N and T = matched samples from non-tumor and tumor tissue, respectively.

Figure 3 Immunohistochemistry for EphA4 in gastric cancer tissues (× 200). A: Well differentiated gastric cancer negative for EphA4; B: Poorly differentiated gastric cancer negative for EphA4; C: Normal gastric epithelia negative for EphA4; D: Moderately differentiated gastric cancer positively stained for EphA4; E: Poorly differentiated gastric cancer positively stained for EphA4.

Figure 4 Kaplan-Meier survival curves of patients with gastric cancer according to the expression of EphA4 (P = 0. 0008).

Figure 5 RT-PCR analysis of the type A ephrins and type B ephrins in gastric cancer cell lines. 1: NUGC3; 2: NUGC4; 3: SNU1; 4: SNU638; 5: MKN28; 6: MKN45; 7: MKN74; 8: KATOIII; 9: HGC27; 10: GC1Y; 11: AZ521.