Effect of Daxx on cholesterol accumulation in hepatic cells

Figure 1 The correlation between Daxx expression of Hepatic tissues and serum cholesterol. A: The effect of control or high fat (HF) food on serum cholesterol and triglycerides in rats; B: Hepatic Daxx expression of rats as estimated by western blotting; C: Quantitative data of Daxx expression, results were normalized to β-actin. Data are the mean ± SE of three independent experiments. Control: normal food. HF: Added high fat to normal food. TC: Total cholesterol; TG: Triglyceride; LDL-C: Low-density lipoprotein cholesterol; HDL-C: High-density lipoprotein cholesterol. ^aP < 0.05 vs control.

Figure 2 Daxx expression in HepG₂ hepatocytes. Cultured HepG₂ cells were untransfected (control, A) or transfected with pEGFP-C1-Daxx or pEGFP-C1 vectors (B, C). Images show the location and expression of Daxx in HepG₂ cells, which were taken at 400 × magnitude. (D) RT-PCR of Daxx mRNA expression.

Figure 3 Effect of Daxx overexpression on cholesterol accumulation in HepG₂ hepatocytes. A: Representative change of intracellular cholesterol levels in HepG₂ cells, as determined by HPLC; B: The contents of free and total cholesterol in HepG₂ cells. FC: Free cholesterol. ^aP > 0.05 vs control; ^cP < 0.05 vs control or HepG₂/GFP cells.

Figure 4 Effect of Daxx on the expression of SREBP and caveolin-1 proteins in HepG₂ hepatocytes. A: Representative western blot data showing the effects of Daxx on SREBP and caveolin-1 proteins in HepG₂ cells; B: Quantitative data of the Daxx effect on SREBP and caveolin-1 expression. Data are the mean ± SE of three independent experiments. ^bP < 0.01 vs control or HepG₂/GFP cells.