Blood F2-isoprostanes are significantly associated with abnormalities of lipid status in rats with steatosis

Figure 1 GC-NICI-MS SIM chromatograms of (A) plasma total iPF_2α-III and (C) hepatic iPF_2α-III isolated from a rat with simple steatosis, and (B) and (D) the internal standard iPF_2α-III-d₄ in the plasma and tissue samples, respectively.

Figure 2 Enhanced lipid accumulation in the liver of rat by high-fat diet. (A) Control, and (B) Steatotic liver (HE, magnification × 100).

Figure 3 Plasma and hepatic levels of iPF_2α-III and antioxidant enzymes in rats. (A) Significant differences in iPF_2α-III levels were found in the liver, but not in plasma between HF rats and control; (B) Significant decreases in SOD activity were found in the liver, but not in erythrocytes of HF rats compared to that in control, and (C) Reducing tendency of CAT activities was observed in liver, but not in blood of HF rats. ^bP < 0.01 and ^dP < 0.001.

Figure 4 The formation of plasma total iPF_2α-III was significantly correlated with the levels of fasting plasma TC (A), TG (B) and ALT (C) in HF rats.