Correlation between expression and differentiation of endocan in colorectal cancer

Figure 1 Expression of endocan in normal mucous membrane and cancer tissues of colon and rectum tissues. A: Endocan was expressed in the cytoplasm of the epithelial cell and it had a high polarity under the nucleus; B: The expression of endocan in colon and rectum tissues. This section show that it had a high expression in mucous membrane by the side of carcinoma tissue (gray arrow), also in well and moderately-differentiated colon carcinoma tissues (black arrow), but a weak expression in poorly differentiated carcinoma tissues (white arrow).

Figure 2 The expression of endocan in normal mucous membrane and different differentiation colon carcinoma tissues. It had a high expression in well and moderately-differentiated colon carcinoma tissues, but a weak expression in poorly differentiated carcinoma tissues. A: The expression of endocan in normal mucous membrane; B: The expression of endocan in well-differentiation colon carcinoma tissue; C: The expression of endocan in well-differentiation colon carcinoma tissue; D: The e x p r e s s i o n o f e n d o c a n in poorly differentiated colon carcinoma tissue; E: Negative control. Left: in situ hybridization; Right: immunohistochemistry.

Figure 3 The expression of endocan in colon and rectum tissues. A: RT-PCR analysis of endocan mRNA in colon and rectum tissues. Endocan mRNA was highly expressed in normal colon and rectum tissue and well and mid- differentiated colorectal cancer tissues, but was down regulated in poorly differentiated colorectal cancer tissues. Endocan expression (up) and β-actin expression (down). 1: 100 bp Marker; 2: Normal colon and rectum mucous membrane; 3: Well-differentiated differentiated colorectal cancer tissue; 4: Moderately colorectal cancer tissue; 5: Poorly differentiated colorectal cancer tissue. B: Expression of endocan by Western blot. Endocan was detected at high expression levels in normal colorectal and well-differentated tissues; Moreover, there was a down regulation in the poorly differentiated colorectal cancer tissues. Lane 1, 3, 5: Normal mocusa, well-differentiated tissues; lane 2, 4, 6: Poorly-differentiated tissues.