PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer

Figure 1 PCR-SSCP showing exons 5 (A) and 7 (B) in PTEN gene. T7, T16, T19 and T37: Positive SSCP; T1: Negative SSCP; T32: Positive SSCP; T31 and T33: Negative SSCP. N: Paracancerous tissue samples; T: Gastric cancer tissue samples.

Figure 2 AA-TCC mutation at 40 bp upstream of 3’ lateral in exon 7 (A), G-C point mutation at 91 bp upstream of 5’ lateral exon 5 (B), T-G point mutation at 24 bp upstream (C) and single base A mutation at 7 bp upstream (D) of 5’ lateral exon 5 in paired paracancerous and gastric cancer tissue samples.